Performance, intestinal histomorphology and bone composition of broiler chickens fed diets supplemented with genistein

The effect of dietary genistein on performance, intestinal morphology, caecal Lactobacillus spp. count, and tibia composition in broiler chickens after 21 and 37 days of feeding was investigated. A total of 360 Cobb 500 broiler chickens (21 days old) were randomly allocated to five treatments with six replicates of 12 birds each. They were fed a basal diet (C) or a basal diet supplemented with 200 (T1), 400 (T2), 600 (T3) and 800 (T4) mg genistein/kg of feed. Genistein supplementation did not affect feed intake, but improved bodyweight, weight gain and feed conversion ratio (FCR) after 21 days, while 600 mg/kg led to a significant increase in FCR after 37 days of feeding. Plasma triglyceride level decreased with dietary genistein after 21 days, while increases were found in T3 and T4 groups after prolonged supplementation. Significantly improved duodenal and jejunal villus length and width, crypt depth and villus/crypt ratio were observed after the first and the second finishing periods, respectively, while adverse effects were found in the ileum for both periods. At 42 days old, greater spleen and heart weights were measured in broilers fed diets with 800 mg/kg than in other broiler groups. The shorter genistein supplementation period (21 days) of 200 and 400 mg/kg had a positive effect on tibia wet weight, ash and calcium (Ca) content, while 37 days of the higher genistein doses administered to the T2, T3 and T4 birds significantly increased caecal lactic acid bacteria (LAB) counts. Thus, recommended doses should not exceed 400 mg/kg. Keywords: broiler performance, blood triglyceride, Lactobacillus, prolonged fattening, small intestinal morpholog

Designing efficient contemporary ship recycling yards through discrete event simulation

Today, the ship recycling industry is going through a transitional phase in order to comply with the new regulations which are forcing ship recycling yard owners to invest and improve yards. One way to compensate these investments is to optimise the current process and increase the efficiency; however, in the literature there is a gap on detailed approach to optimization of ship recycling processes. This paper aims to develop a framework for the ship recycling industry in order to improve and optimise the ship recycling procedures. This aim is achieved through preparation of simulation models in the ARENA software with current and alternatives practices/processes for every step of ship recycling. The simulation framework developed has been applied for a ship recycling yard in EU as a case study. Bottlenecks in the current process were identified and through simulations alternative solutions were considered to optimise the process. Potential improvements for the yard with simulation approach were summarized in this paper

A study on evaluating the status of current occupational training in the ship recycling industry in Bangladesh

Essential to steel production in Bangladesh, the ship recycling industry has become a vital component of the country’s economy. After four decades of growth, Bangladesh continues to compete with India to lead the international ship recycling industry. In the Chittagong area, the ship recycling sector, and related downstream activities, provides employment and resources to nearby communities. Despite its profitability and income generation, poor working practices and low environmental standards tarnish the image of the sector at national and international levels. Numerous accidents and fatalities in ship recycling processes highlight the lack of adequate Occupational Health and Safety (OHS) standards. Seasonal migrant workers, many of whom unfamiliar with industrial settings, represent the majority of workers in ship recycling yards. Recruited by subcontractors, most of the workers in Bangladesh lack sufficient hazard awareness and basic safety requirements necessary to work in a ship recycling yard. For these reasons, as identified by the Bangladesh High Court and subsequent national rules, training of workers must be addressed in yards in Bangladesh. In this study, associated with the IMO-NORAD SENSREC project, existing conditions, solely about workers’ training, has been addressed, assessed, and compared in accordance with IMO’s Hong Kong Convention and ILO requirements, where detailed recommendations were provided to enhance OHS training in the yards

An approach to advance circular practices in the maritime industry through a database as a bridging solution

The concept of maritime circularity has gained increasing attention to address challenges arising from the net-zero targets of the maritime industry. The circular economy provides potential solutions to address these challenges through reuse, remanufacturing, and recycling practices. However, the industry faces complex challenges, including inefficient reverse supply chains, a lack of awareness about circular economy principles, standardisation issues, and the need for digital infrastructure to provide vital information in the sector. These challenges prevent the implementation of circularity practices, as access to crucial data throughout the vessel’s life cycle is obstructed. This novel research aims to create a robust first-of-its-kind database solution specifically designed to support the industry’s shift towards circularity. The database will facilitate fast and transparent information flow between the stakeholders, providing foundations for asset tracking and a robust reverse supply chain. A case study was conducted to show that a database could help extract higher financial value from end-of-life ships by over 80%. The ageing fleet increases the urgency of utilising such a database, which could be a pivotal strategy for a sustainable and circular industry. This digital solution offers significant benefits to all industry stakeholders and allows holistic resource management, influencing maritime operations’ sustainability, resilience, and profitability

Towards a circular maritime industry : identifying strategy and technology solutions

Shipping is considered one of the most energy-efficient modes, considering the amount of cargo that can be carried. On the other hand, the circular economy approach is not well-established in the maritime industry, which currently lags behind different transport modes. The maritime industry needs scientific support to “close the loop”, minimise waste and increase the revenue stream. Therefore, this study aims to address a critical gap in the maritime industry by first showing the understanding of the stakeholders and identifying suitable strategy and technology solutions that can fit the characteristics of the maritime industry. Moreover, the potential benefits of these solutions have been demonstrated through high-speed marine engine remanufacturing. A cost-benefit analysis has shown that remanufactured engine acquisition cost is nearly half of the cost of a new engine with similar operating performance and operating cost. This study is a novel contribution to maritime industry stakeholders to demonstrate the advantages of circular end-of-life applications

Workers' exposure to dust and potentially toxic elements during steel cutting in two ship dismantling cases

Ship dismantling is the recommended recycling solution for the end of a ship’s life, but the process is not free of occupational risk. Despite proper regulations, there are underlying chemical and physical hazards, mainly due to the cutting of steel parts, which is the core of the recycling process. The overall aim of this research study is to determine, in two case study examples, the ship recycling workers’ potential occupational exposure by inhalation of chemical agents generated by the torch cutting process of coated and de-coated steel. This was carried out specifically through (i) monitoring and measuring ship recycling workers’ local environment for the inhalable (total dust) and respirable (fine dust) fractions during their working operations, (ii) analysing the heavy metal content of the dust and (iii) calculating and comparing this against occupational exposure limits, (v) comparing de-coating operations with cutting of coated and de-coated steel. Results of this study show that without further mitigation workers involved in torch cutting processes are at high risk of exposure to heavy metals by inhalation as these are exceeding the norms defined by regulatory bodies

Human small intestinal infection by SARS-CoV-2 is characterized by a mucosal infiltration with activated CD8+ T cells

The SARS-CoV-2 pandemic has so far claimed over three and a half million lives worldwide. Though the SARS-CoV-2 mediated disease COVID-19 has first been characterized by an infection of the upper airways and the lung, recent evidence suggests a complex disease including gastrointestinal symptoms. Even if a direct viral tropism of intestinal cells has recently been demonstrated, it remains unclear, whether gastrointestinal symptoms are caused by direct infection of the gastrointestinal tract by SARS-CoV-2 or whether they are a consequence of a systemic immune activation and subsequent modulation of the mucosal immune system. To better understand the cause of intestinal symptoms we analyzed biopsies of the small intestine from SARS-CoV-2 infected individuals. Applying qRT-PCR and immunohistochemistry, we detected SARS-CoV-2 RNA and nucleocapsid protein in duodenal mucosa. In addition, applying imaging mass cytometry and immunohistochemistry, we identified histomorphological changes of the epithelium, which were characterized by an accumulation of activated intraepithelial CD8(+) T cells as well as epithelial apoptosis and subsequent regenerative proliferation in the small intestine of COVID-19 patients. In summary, our findings indicate that intraepithelial CD8(+) T cells are activated upon infection of intestinal epithelial cells with SARS-CoV-2, providing one possible explanation for gastrointestinal symptoms associated with COVID-19

Optical gain in 1.3-μm electrically driven dilute nitride VCSOAs

We report the observation of room-temperature optical gain at 1.3 μm in electrically driven dilute nitride vertical cavity semiconductor optical amplifiers. The gain is calculated with respect to injected power for samples with and without a confinement aperture. At lower injected powers, a gain of almost 10 dB is observed in both samples. At injection powers over 5 nW, the gain is observed to decrease. For nearly all investigated power levels, the sample with confinement aperture gives slightly higher gain

Structure, mechanism, and inhibition of Hedgehog acyltransferase

The Sonic Hedgehog (SHH) morphogen pathway is fundamental for embryonic development and stem cell maintenance and is implicated in various cancers. A key step in signaling is transfer of a palmitate group to the SHH N terminus, catalyzed by the multi-pass transmembrane enzyme Hedgehog acyltransferase (HHAT). We present the high-resolution cryo-EM structure of HHAT bound to substrate analog palmityl-coenzyme A and a SHH-mimetic megabody, revealing a heme group bound to HHAT that is essential for HHAT function. A structure of HHAT bound to potent small-molecule inhibitor IMP-1575 revealed conformational changes in the active site that occlude substrate binding. Our multidisciplinary analysis provides a detailed view of the mechanism by which HHAT adapts the membrane environment to transfer an acyl chain across the endoplasmic reticulum membrane. This structure of a membrane-bound O-acyltransferase (MBOAT) superfamily member provides a blueprint for other protein-substrate MBOATs and a template for future drug discovery

Expanding the clinical and immunological phenotypes of PAX1-deficient SCID and CID patients

Paired box 1 (PAX1) deficiency has been reported in a small number of patients diagnosed with otofaciocervical syndrome type 2 (OFCS2). We described six new patients who demonstrated variable clinical penetrance. Reduced transcriptional activity of pathogenic variants confirmed partial or complete PAX1 deficiency. Thymic aplasia and hypoplasia were associated with impaired T cell immunity. Corrective treatment was required in 4/6 patients. Hematopoietic stem cell transplantation resulted in poor immune reconstitution with absent naïve T cells, contrasting with the superior recovery of T cell immunity after thymus transplantation. Normal ex vivo differentiation of PAX1-deficient CD34+ cells into mature T cells demonstrated the absence of a hematopoietic cell-intrinsic defect. New overlapping features with DiGeorge syndrome included primary hypoparathyroidism (n = 5) and congenital heart defects (n = 2), in line with PAX1 expression during early embryogenesis. Our results highlight new features of PAX1 deficiency, which are relevant to improving early diagnosis and identifying patients requiring corrective treatment